The SarA protein family of Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Global regulation of virulence determinants in Staphylococcus aureus by the SarA protein family.
In S. aureus, the production of virulence determinants such as cell wall adhesins and exotoxins during the growth cycle is controlled by global regulators such as SarA and agr. Genomic scan reveals 16 two-component regulatory systems (e.g. agr and sae) as well as a family of SarA homologs in S. aureus. We call the SarA homologs the SarA protein family. Many of the members in this protein family...
متن کاملCharacterization of Staphylococcus aureus SarA binding sites.
The staphylococcal accessory regulator locus (sarA) encodes a DNA-binding protein (SarA) that modulates expression of over 100 genes. Whether this occurs via a direct interaction between SarA and cis elements associated with its target genes is unclear, partly because the definitive characteristics of a SarA binding site have not been identified. In this work, electrophoretic mobility shift ass...
متن کاملSarA of Staphylococcus aureus binds to the sarA promoter to regulate gene expression.
The 375-bp sarA open reading frame is driven by three promoters, P1, P3, and P2. Using gel shift and DNase I footprinting assays, we found that SarA binds to two 26-bp sequences and one 31-bp sequence within the P1 and P3 promoters, respectively. Together with the results of transcription analyses, our data indicate that SarA binds to its own promoter to down-regulate sarA expression.
متن کاملRole of the distal sarA promoters in SarA expression in Staphylococcus aureus.
The global regulatory locus sarA comprises a 375-bp open reading frame that is driven by three promoters, the proximal P1 and distal P3 and P2 promoters. We mutated the weaker P3 and P2 promoters to ascertain the effect of the change on SarA protein and target gene expression. Our results indicated that the solely active P1 promoter led to a lower SarA protein level, which has an effect on agr ...
متن کاملMutation of sarA in Staphylococcus aureus limits biofilm formation.
Mutation of sarA resulted in a reduced capacity to form a biofilm in six of the eight Staphylococcus aureus strains we tested (UAMS-1, UAMS-601, SA113, SC-01, S6C, and DB). The exceptions were Newman, which formed a poor biofilm under all conditions, and RN6390, which consistently formed a biofilm only after mutation of agr. Mutation of agr in other strains had little impact on biofilm formatio...
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ژورنال
عنوان ژورنال: The International Journal of Biochemistry & Cell Biology
سال: 2008
ISSN: 1357-2725
DOI: 10.1016/j.biocel.2007.10.032